- Microorganisms are abundant and found in food stuff as well.
- The microbes found on food can be pathogenic in nature and may cause various diseases.
- The identification of such microbes is necessary to study and research on infectious agent.
- Primary culture from food stuff is a mix culture of various microbes which has to be isolate form each other and identify for research.
- The procedure below is used to isolate and cultivate pure culture form food stuffs.
- Nutrient agar plate
- Food sample
- Test tube
- Take nutrient agar plate and well label them.
- In one test tube take 4.5 ml autoclaved water and add small quantity of food sample in it
- Mix thoroughly, after that with the help of micropipette take 100 microliter of the mixture and spread onto the petri plate with a spreader.
- Finally put the petri plates in the incubator for 18-24 hours at 37o C.
- After the incubation period mark the different colonies with marker.
- Now pick each single colony with an inoculating loop and streak on nutrient agar plate.
- Put the petri plates inside the incubator for 18- 24 hours at 37o C.
- After 18-24 hours examine the plates for bacterial growth.
- Record the result of isolated colonies in tabular form.