Isolation And Culturing Of Microbes From Food

Theory:

• Microorganisms are abundant and found in food stuff as well.
• The microbes found on food can be pathogenic in nature and may cause various diseases.
• The identification of such microbes is necessary to study and research on infectious agent.
• Primary culture from food stuff is a mix culture of various microbes which has to be isolate form each other and identify for research.
• The procedure below is used to isolate and cultivate pure culture form food stuffs.

Requirements:

• Nutrient agar plate
• Food sample
• Spreader
• Micropipette
• Test tube

Procedure:

• Take nutrient agar plate and well label them.
• In one test tube take 4.5 ml autoclaved water and add small quantity of food sample in it
• Mix thoroughly, after that with the help of micropipette take 100 microliter of the mixture and spread onto the petri plate with a spreader.
• Finally put the petri plates in the incubator for 18-24 hours at 37^o C.
• After the incubation period mark the different colonies with marker.
• Now pick each single colony with an inoculating loop and streak on nutrient agar plate.
• Put the petri plates inside the incubator for 18- 24 hours at 37^o C.

Observations:

• After 18-24 hours examine the plates for bacterial growth.

Result:

• Record the result of isolated colonies in tabular form.